L. GALATEANU, M. BAZU, V. ILIAN, C. TIBEICA, N. CIMPOCA, C. PODARU, I.
ARDELEAN, L. DUMITRU, M. GRIGORAS, M. IVANOIU, M. TOTOLIN, D. CONDURUTA
Electrochemical Micro-Cell for (Cyano)Bacteria-Based Biosensors
An electrochemical micro-cell for building micro-biosensors based on detection
of photosynthesis inhibition for cyanobacteria was achieved. A system of three
micro-electrodes was realized on a Si chip, using the photolithographic
techniques of planar technologies for semiconductor devices. The Synechocystis
PCC 6803, MscL mutant, was selected as biological element having a high
sensitivity for xenobiotics. A polypyrrole layer deposited on the working
electrode by electrochemical polymerization assured the interface with the
biological element. Constructive solutions where adopted to fulfil the multiple
phenomena interference requirements. The work was carried on in the frame of
BIOXEN project, supported by MATNANTECH National R&D Programme.